| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.064274 |
| Chromosome: | chromosome 15 |
| Location: | 99095 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g634827 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGCCATCAAGAATTTTCAGTTTTATGG |
| Internal bar code: | TGCGGCCGTGTGAGAAAGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1428 |
| LEAP-Seq percent confirming: | 81.5444 |
| LEAP-Seq n confirming: | 4224 |
| LEAP-Seq n nonconfirming: | 956 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAATTGTTTGGGCACGAAG |
| Suggested primer 2: | ACCTCCACATCCTACGCAAC |