| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.064279 |
| Chromosome: | chromosome 6 |
| Location: | 452904 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g252550 | DLA3 | Dihydrolipoamide acetyltransferase; (1 of 1) PTHR23151//PTHR23151:SF56 - DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED // DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT 1 OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGACGAGGGCTTTGTTGCAAAGCTTCTT |
| Internal bar code: | CCATGCACGTCTACCTCACTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 915 |
| LEAP-Seq percent confirming: | 99.1404 |
| LEAP-Seq n confirming: | 1730 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCTGTGTGGTTGATAGG |
| Suggested primer 2: | AACCTCGACTCAGACACGCT |