| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.064307 |
| Chromosome: | chromosome 16 |
| Location: | 4550676 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTACTACAGCATCCGCGCCTTTTCAGGTT |
| Internal bar code: | AGCTGGGATTGCATTTTATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1159 |
| LEAP-Seq percent confirming: | 96.6684 |
| LEAP-Seq n confirming: | 1828 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGCATGCATGACTGTGA |
| Suggested primer 2: | GAGGAAGAGGCACTGACAGG |