| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.064321 |
| Chromosome: | chromosome 12 |
| Location: | 9173084 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542150 | (1 of 3) PF14108 - Domain of unknown function (DUF4281) (DUF4281) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCTTCCAACGCGGGAGCAGTGCAAGTA |
| Internal bar code: | AGTAAAGTCGGCGGCCGATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 939 |
| LEAP-Seq percent confirming: | 99.5059 |
| LEAP-Seq n confirming: | 3222 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCATCATGCACAGGAACA |
| Suggested primer 2: | CGTAGCCAGCTCCATCTAGG |