| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064350 |
| Chromosome: | chromosome 14 |
| Location: | 1871399 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620652 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCTGGAGTGCGTGTGAGGAAGGGGAGGG |
| Internal bar code: | CGGACGATTCACCATGGCTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 88.7709 |
| LEAP-Seq n confirming: | 3763 |
| LEAP-Seq n nonconfirming: | 476 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACGAGCACTATCACTCCG |
| Suggested primer 2: | GGCCTTTACTACCGACACCA |