| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.064350 |
| Chromosome: | chromosome 14 |
| Location: | 1871400 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620652 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTGTCTGGCGGCGGCGGCGGCGGCTCC |
| Internal bar code: | GTGGGCGGTCCTGGCCGCAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 87.0221 |
| LEAP-Seq n confirming: | 6578 |
| LEAP-Seq n nonconfirming: | 981 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTTTACTACCGACACCA |
| Suggested primer 2: | TGACGAGCACTATCACTCCG |