| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.064414 |
| Chromosome: | chromosome 8 |
| Location: | 4135008 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379850 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGTCTCGGAGCGGGGCCATGCGTGGGA |
| Internal bar code: | TCGCGGTGGGCCACAGCGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 812 |
| LEAP-Seq percent confirming: | 99.7136 |
| LEAP-Seq n confirming: | 3482 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAACCTTGCTATCTAGCCG |
| Suggested primer 2: | ACTGCTCCACCTCTTCCTCA |