| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064454 |
| Chromosome: | chromosome 3 |
| Location: | 6619027 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196600 | RAD17 | (1 of 1) PTHR12172//PTHR12172:SF0 - CELL CYCLE CHECKPOINT PROTEIN RAD17 // RAD17; presequence translocase-associated protein import motor subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGGGATGGTGATGACATCTGACGTCAA |
| Internal bar code: | GGGGACACCGTAAGCGAATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 969 |
| LEAP-Seq percent confirming: | 83.8828 |
| LEAP-Seq n confirming: | 229 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGTGCATGAGGTTACAG |
| Suggested primer 2: | CCAGAGCACTAGGACGAAGG |