| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.064460 |
| Chromosome: | chromosome 8 |
| Location: | 4132661 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379800 | (1 of 1) PF00207//PF07974 - Alpha-2-macroglobulin family (A2M) // EGF-like domain (EGF_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCCGGAGCGGAGTGAGGCGTAGGAGAA |
| Internal bar code: | GTGCCAGGCTGATGAGAGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 94.436 |
| LEAP-Seq n confirming: | 2495 |
| LEAP-Seq n nonconfirming: | 147 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTAAGCCGTCCAGCTAAG |
| Suggested primer 2: | ATGTAACGCAGTTTCCCGAC |