| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.064474 |
| Chromosome: | chromosome 7 |
| Location: | 6063233 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355250 | WEE1 | CDK inhibitory kinase; (1 of 1) K06632 - wee1-like protein kinase (WEE1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCCCGAACTTTCTCACCACACCACGG |
| Internal bar code: | GGTATCGGTTTGCGACGGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 694 |
| LEAP-Seq percent confirming: | 99.4048 |
| LEAP-Seq n confirming: | 501 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTAGGTGAAGGGAGCAGC |
| Suggested primer 2: | GAGGCCTCAGTTGGCTGTAG |