| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.064477 |
| Chromosome: | chromosome 12 |
| Location: | 8405764 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548700 | 3'UTR_intron|outside_mRNA | ||
| Cre12.g548750 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGACCCGAGCTCCCGCCCCTCCTTCCCC |
| Internal bar code: | CGATGTGTGAGCGGGATTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 98.5714 |
| LEAP-Seq n confirming: | 138 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGTGCCTTTCAAGTAGC |
| Suggested primer 2: | GTGCCTCTGGAGGTCTTCTG |