| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.064488 |
| Chromosome: | chromosome 2 |
| Location: | 929471 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079700 | PYR2 | (1 of 1) 2.1.3.2 - Aspartate carbamoyltransferase / Carbamylaspartotranskinase; Aspartate carbamoyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGTGTATGTGCGCGTGTGCGGTTGGG |
| Internal bar code: | GACATACACCTCGTATGACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 82.9052 |
| LEAP-Seq n confirming: | 4583 |
| LEAP-Seq n nonconfirming: | 945 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCCTCTTGCTTTCCTGA |
| Suggested primer 2: | TCAACACAATTTTGGCGTGT |