| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064493 |
| Chromosome: | chromosome 3 |
| Location: | 6009432 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190900 | (1 of 5) PTHR10351:SF42 - LD40663P | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATGGTGGTGTGGGCCGCAATAATGAGC |
| Internal bar code: | GCCCGATGACGCATGAACAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 897 |
| LEAP-Seq percent confirming: | 97.4036 |
| LEAP-Seq n confirming: | 2476 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCTTACCCTGTCCAATC |
| Suggested primer 2: | TAGGCAAGATAGGCAGCGTT |