| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064531 |
| Chromosome: | chromosome 10 |
| Location: | 5301880 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457500 | CKIN beta,SNRKB1 | regulatory subunit beta of snRK1 complex in Chlamydomonas; (1 of 1) K07199 - 5'-AMP-activated protein kinase, regulatory beta subunit (PRKAB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTACGGTACGATGGAGGCACAGATGGAGT |
| Internal bar code: | GAGTATACTCGAGCGCCTCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 784 |
| LEAP-Seq percent confirming: | 73.6403 |
| LEAP-Seq n confirming: | 1855 |
| LEAP-Seq n nonconfirming: | 664 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTGTGTGAGGTGGAATG |
| Suggested primer 2: | ACACTGCGTGACAGCGTATC |