| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064538 |
| Chromosome: | chromosome 3 |
| Location: | 3096824 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164750 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCGACTGAGAGTGATTTGGGTTGTTGG |
| Internal bar code: | GCATGAGGGTAAAGCGACGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 97.5418 |
| LEAP-Seq n confirming: | 2857 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGAACCCATGTAACCCAT |
| Suggested primer 2: | GAATCTAAAGCAACGCAGGC |