Insertion junction: LMJ.RY0402.064543_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.064543
Chromosome:chromosome 16
Location:563433
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g692450 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGGACACCAGGTACACCCCCGCGCCCCG
Internal bar code:GCACGAACAGCGTATGTCCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:204
LEAP-Seq percent confirming:98.1308
LEAP-Seq n confirming:105
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTTATCCCCCAAAGGACCAT
Suggested primer 2:TGTAGGTGGACTGGAGGAGG