| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064549 |
| Chromosome: | chromosome 8 |
| Location: | 2514045 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g372000 | BAF3,CGLD6,CGLD11 | chloroplast ATPase CF1 assembly factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGAACGGCAGTGGTGCAAAAGCAGTTCT |
| Internal bar code: | GCGGGCCTAGGGAGACATTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 908 |
| LEAP-Seq percent confirming: | 87.9329 |
| LEAP-Seq n confirming: | 11579 |
| LEAP-Seq n nonconfirming: | 1589 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTCCATACACATGCAC |
| Suggested primer 2: | ATCCTTTGCTCGCTTTGAGA |