| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.064584 |
| Chromosome: | chromosome 2 |
| Location: | 3553177 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095129 | (1 of 1) PTHR23257//PTHR23257:SF537 - SERINE-THREONINE PROTEIN KINASE // PROTEIN KINASE FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAATGTACCCCACCCACCCCCCGTCTTG |
| Internal bar code: | AGGTAAAGTGCTGCTTGATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1045 |
| LEAP-Seq percent confirming: | 99.624 |
| LEAP-Seq n confirming: | 3444 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCGGTGGAGATAGAGAG |
| Suggested primer 2: | GCAAGTACCTGGCTTCAACC |