Insertion junction: LMJ.RY0402.064590_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.064590
Chromosome:chromosome 11
Location:845265
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467649 OPR107 OctotricoPeptide Repeat protein 107; (1 of 1) IPR010622//IPR016024 - FAST kinase leucine-rich // Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCAATAACAGCAGGCCCGGTCATGGGCGC
Internal bar code:GATGGAGGCTTCTAAAACACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:47
LEAP-Seq percent confirming:1.29505
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:1753
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAGCATGCGTGACTTGAA
Suggested primer 2:GCAAGACTCCAGTACGCTCC