Insertion junction: LMJ.RY0402.064593_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.064593
Chromosome:chromosome 16
Location:3881495
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g690150 ALG6,GTR21 (1 of 1) 2.4.1.267 - Dolichyl-P-Glc:Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase / Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1->3-glucosyltransferase; Alpha-1%252C3-glucosyltransferase 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTTGGCTGCGTCATGGCGCATGTATAAA
Internal bar code:ACGAGCGCGCGGTGGCAGCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:140
LEAP-Seq percent confirming:98.9005
LEAP-Seq n confirming:1799
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GACACAGCCTCGAAGACACA
Suggested primer 2:GCCATGTGTTTGTGGTTGAG