| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.064664 |
| Chromosome: | chromosome_1 |
| Location: | 6675763 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g047950 | FAP156,IFT27 | Intraflagellar transport protein 27 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACAGTCCAAGGGCTTCATGTACATAGACTG |
| Internal bar code: | TTCTCGAACCACGGGAGATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 92.5843 |
| LEAP-Seq n confirming: | 412 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTTGTTGCGGTGTATGAC |
| Suggested primer 2: | TTCTCGTGTTCGATGTGAGC |