| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.064695 |
| Chromosome: | chromosome 9 |
| Location: | 5204097 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399326 | MITC3, MCP3 | (1 of 2) K15110 - solute carrier family 25 (mitochondrial 2-oxodicarboxylate transporter), member 21 (SLC25A21, ODC); Mitochondrial substrate carrier protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGTTGGTCGGCGACATTTCCGATAGCGC |
| Internal bar code: | TCCTCTACGGTTACTAGTGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 709 |
| LEAP-Seq percent confirming: | 99.7451 |
| LEAP-Seq n confirming: | 16045 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCCCTGTTGTCGTTCTGT |
| Suggested primer 2: | GAGCCTGTTGAGTTTCGGAG |