| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064710 |
| Chromosome: | chromosome 15 |
| Location: | 870795 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639503 | GEX173 | (1 of 7) PTHR23257//PTHR23257:SF474 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCTGTCCTCACTCACTTCACTTTCCGGG |
| Internal bar code: | GGGCCCGAGGTAAGGGCTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 99.3823 |
| LEAP-Seq n confirming: | 4183 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACACTGAACCAAACCCAA |
| Suggested primer 2: | ACCATAGGTTTGGAGGGGTC |