Insertion junction: LMJ.RY0402.064718_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.064718
Chromosome:chromosome 14
Location:2485989
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g625050 (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGGCCCACTCACCTTCAGGTCCCTGTG
Internal bar code:CGGATTCAGCACTGTCGCGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:370
LEAP-Seq percent confirming:97.3435
LEAP-Seq n confirming:513
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCTTCCCTGACAACATCC
Suggested primer 2:CCCCACACTGTAGGCAATCT