| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064726 |
| Chromosome: | chromosome 12 |
| Location: | 2458128 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506850 | (1 of 2) PTHR10183//PTHR10183:SF323 - CALPAIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACAAGCGTCCCACACTTGACCGCTGCC |
| Internal bar code: | TCGATAATGCGGCTACTGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 835 |
| LEAP-Seq percent confirming: | 97.5019 |
| LEAP-Seq n confirming: | 20335 |
| LEAP-Seq n nonconfirming: | 521 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAGGAGTGTTGAGTGGC |
| Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |