Insertion junction: LMJ.RY0402.064730_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.064730
Chromosome:chromosome 2
Location:7284461
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g145500 PTK24 (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; Protein tyrosine kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGCGTGCGAACTGGAGCTGGTCGATGAT
Internal bar code:CGTCACCGACAACTGGGGCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:784
LEAP-Seq percent confirming:98.9285
LEAP-Seq n confirming:3693
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGAAGACGTGCTGTGTAT
Suggested primer 2:ACGTGCGCTAAACCCAATAC