Insertion junction: LMJ.RY0402.064738_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.064738
Chromosome:chromosome 7
Location:4226059
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g342000 (1 of 1) PF00651//PF12847 - BTB/POZ domain (BTB) // Methyltransferase domain (Methyltransf_18) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACGCAACCACCCGCCCACATATGCAATTG
Internal bar code:CCCTGTTATGTCCAGGCGAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:632
LEAP-Seq percent confirming:99.5214
LEAP-Seq n confirming:4575
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGTCCACCATCCGAGTTG
Suggested primer 2:GCACACACACCAATCACACA