| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.064738 |
| Chromosome: | chromosome 7 |
| Location: | 4226072 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342000 | (1 of 1) PF00651//PF12847 - BTB/POZ domain (BTB) // Methyltransferase domain (Methyltransf_18) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCGGGCGTACGGCACCATGTGAGGTAG |
| Internal bar code: | GTGGTTGAAAGTTTGTTGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 320 |
| LEAP-Seq percent confirming: | 99.6916 |
| LEAP-Seq n confirming: | 15516 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTCCACCATCCGAGTTG |
| Suggested primer 2: | GCACACACACCAATCACACA |