| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.064751 |
| Chromosome: | chromosome 2 |
| Location: | 8585755 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141986 | (1 of 2) IPR000104//IPR000719//IPR001245//IPR011009 - Antifreeze protein, type I // Protein kinase domain // Serine-threonine/tyrosine-protein kinase catalytic domain // Protein kinase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCCCTGGCCTGAGTCTGCAGCACGCAG |
| Internal bar code: | CGAAGGCAGTACACCATGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 95.4846 |
| LEAP-Seq n confirming: | 867 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGCAGACCCACAGCAGA |
| Suggested primer 2: | CTCGCATTACTCGCATCTCA |