Insertion junction: LMJ.RY0402.064753_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.064753
Chromosome:chromosome 13
Location:4534406
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g602750 (1 of 5) PTHR32467:SF15 - AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR SMZ-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGCCCCACGCATCCACGGCCTCTCGGTC
Internal bar code:TCAATATGATCTGCTCAATAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:615
LEAP-Seq percent confirming:98.2003
LEAP-Seq n confirming:18006
LEAP-Seq n nonconfirming:330
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTCTCTCAACAGCACCCA
Suggested primer 2:GGGCTCGGACACACACTTAT