| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.064782 |
| Chromosome: | chromosome 11 |
| Location: | 1054515 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467673 | PDE24 | (1 of 17) 3.1.4.53 - 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCATTACATGCCAGCGACGAGATCACC |
| Internal bar code: | TTAATTACGAGGCTAACTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 375 |
| LEAP-Seq percent confirming: | 99.6545 |
| LEAP-Seq n confirming: | 10384 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGAGTTCACTCGGGGTAA |
| Suggested primer 2: | ACCTTTGCAACCTGTATCCG |