Insertion junction: LMJ.RY0402.064785_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.064785
Chromosome:chromosome 4
Location:1172013
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g215700 (1 of 4) IPR009030//IPR011641 - Insulin-like growth factor binding protein, N-terminal // Tyrosine-protein kinase ephrin type A/B receptor-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGCACCTATTCGGACACATACGGCACTC
Internal bar code:GCTGTGAGACGGACGGACACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:299
LEAP-Seq percent confirming:99.1266
LEAP-Seq n confirming:227
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCACTCCAGCTGAACCA
Suggested primer 2:TGTCGTACGTAACTGCCAGC