| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064803 |
| Chromosome: | chromosome 16 |
| Location: | 7708914 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690991 | weak Similarity to Elongation factor 1 | gamma chain; (1 of 1) K03233 - elongation factor 1-gamma (EEF1G) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATACATTGCCTGCGCGCGGCATTCGCAAG |
| Internal bar code: | ACTCGAACCGTCTGTATGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 96.683 |
| LEAP-Seq n confirming: | 4547 |
| LEAP-Seq n nonconfirming: | 156 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAGCTCATCATCCCAAG |
| Suggested primer 2: | CTCTTTGAGGTTCCTGGCTG |