| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.064823 |
| Chromosome: | chromosome_6 |
| Location: | 2174654 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g265950 | DHC3 | Flagellar inner arm dynein heavy chain, novel minor type dynein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGGGGTGGGAGTGTTGTTGACTTTGGTGTT |
| Internal bar code: | TGACGCAAGGGTGTGCTAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 98.6021 |
| LEAP-Seq n confirming: | 30894 |
| LEAP-Seq n nonconfirming: | 438 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGAAAGGAATGCTTGGGT |
| Suggested primer 2: | ACACCTTTCCGCAACCTATG |