Insertion junction: LMJ.RY0402.064829_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.064829
Chromosome:chromosome 13
Location:779310
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g566900 (1 of 1) PTHR11289:SF0 - BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGTGGCCTGCAGCAACTACCCGTGCGTA
Internal bar code:GCCCGTGACTTGGTAGGTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:248
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:137
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAAGAGCACAGGTGGCTA
Suggested primer 2:GCGATAAAAGGAACAGCAGC