| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.064847 |
| Chromosome: | chromosome 11 |
| Location: | 2386103 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g474700 | BES6 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); putative chloride channel | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAACCAGATACCGGCCTGTACCGGCCG |
| Internal bar code: | CAGGAAATCTAGGCTGGATCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1162 |
| LEAP-Seq percent confirming: | 99.4616 |
| LEAP-Seq n confirming: | 7574 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATGTATGCGCTCGTCAGC |
| Suggested primer 2: | ACACACACACACACACACCG |