| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.064850 |
| Chromosome: | scaffold 26 |
| Location: | 61074 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre26.g756997 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGCCCACGCAGTGCGGTGCGGTGCCA |
| Internal bar code: | AGCTAGTCACGGGTCCCAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 80.0165 |
| LEAP-Seq n confirming: | 3888 |
| LEAP-Seq n nonconfirming: | 971 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGTGTGTATGTGGCCTT |
| Suggested primer 2: | GTGGCTCCTTCGCTTTGTAG |