| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.064896 |
| Chromosome: | chromosome 10 |
| Location: | 4707225 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453600 | DES,FAD13 | (1 of 2) PTHR19353:SF30 - FATTY ACID DESATURASE 2; Fatty acid omega 13 desaturase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTTCGCAAGCCCGGAGCCGCCGCTACC |
| Internal bar code: | TCCTGGCACAACTCTCGTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 99.7668 |
| LEAP-Seq n confirming: | 2567 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGCCCCAAACCATCTTCT |
| Suggested primer 2: | AGCACAGACACACACCTTCG |