| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064909 |
| Chromosome: | chromosome 10 |
| Location: | 4080368 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449650 | (1 of 4) IPR001810//IPR020683 - F-box domain // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCCACAGGTCCGCCAGAACGCCCGCCT |
| Internal bar code: | CCCTTTAGCGTGGACTGGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 98.3173 |
| LEAP-Seq n confirming: | 409 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGCACACACGTGGATTT |
| Suggested primer 2: | CTTCCTGAAAGCGGCACTAC |