| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.064918 |
| Chromosome: | chromosome 16 |
| Location: | 813012 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647602 | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGCGAAGAAGGCGGGTGGGAAGGGCG |
| Internal bar code: | CTAGTTGTGTCCGGTTGCACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 97.3626 |
| LEAP-Seq n confirming: | 3544 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | GGCATGTACGGAACCTCAGT |