Insertion junction: LMJ.RY0402.064927_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.064927
Chromosome:chromosome 15
Location:1696487
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre15.g644051 (1 of 7) PF12680 - SnoaL-like domain (SnoaL_2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGTATCTACAACCCCCACGGTCCGCTTA
Internal bar code:GCCACGCGTAAGTAATCACGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:857
LEAP-Seq percent confirming:99.5257
LEAP-Seq n confirming:36718
LEAP-Seq n nonconfirming:175
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAAGTAGCGATTTTCCGC
Suggested primer 2:GAACACCGGTAAAGCGGTTA