Insertion junction: LMJ.RY0402.064974_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.064974
Chromosome:chromosome 16
Location:3683448
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCGACTGACCCTCACCACCGTGCAACCC
Internal bar code:CTGAGTCGTGGAAGGGTAGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:260
LEAP-Seq percent confirming:51.7454
LEAP-Seq n confirming:252
LEAP-Seq n nonconfirming:235
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCCCTGTTGCACGTATCC
Suggested primer 2:CCCTAAACGCCCCTCTAAAC