| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.064980 |
| Chromosome: | chromosome 12 |
| Location: | 5679778 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532450 | (1 of 3) 1.1.1.22 - UDP-glucose 6-dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCCGCGCGCTCCTCCACGTCCACGTCC |
| Internal bar code: | ATTTAACAGTATGAAAGAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 354 |
| LEAP-Seq percent confirming: | 99.6186 |
| LEAP-Seq n confirming: | 1567 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGCTGCCATTTTTAAGC |
| Suggested primer 2: | GCTGCTGCTGTTACACCTGA |