Insertion junction: LMJ.RY0402.064992_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.064992
Chromosome:chromosome 6
Location:8578021
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g308650 (1 of 1) IPR003072//IPR003980//IPR005404 - Orphan nuclear receptor, NOR1 type // Histamine H3 receptor // Potassium channel, voltage dependent, Kv3.3 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGGCAGCTGAGCGAGGACTGGCCCACCG
Internal bar code:CTGTCATCGTCAATTCCACTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:236
LEAP-Seq percent confirming:99.5037
LEAP-Seq n confirming:401
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAGCGTCAACTACACCCA
Suggested primer 2:GCGGCTTTGAAGTTCTCAAC