Insertion junction: LMJ.RY0402.065040_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.065040
Chromosome:chromosome 1
Location:1633384
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g008600 (1 of 1) IPR017993//IPR022751//IPR029044 - Atrophin-1 // Alpha-mannosyltransferase // Nucleotide-diphospho-sugar transferases CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACTATGGTGACGTCGGCCGCTCGGACGA
Internal bar code:GATCGGGCAGACATTCCTGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:322
LEAP-Seq percent confirming:66.0194
LEAP-Seq n confirming:2516
LEAP-Seq n nonconfirming:1295
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AATACTACGTGCGTCCCACC
Suggested primer 2:AACGTGCGTGTGTTTGTGTT