| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065051 |
| Chromosome: | chromosome 14 |
| Location: | 2945157 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627650 | FAP392 | Flagellar Associated Protein 392 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAAGTGTCGCGAAGTAGACAGGGGGTGG |
| Internal bar code: | TTGGACTTCACGGTCTGGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 99.586 |
| LEAP-Seq n confirming: | 7456 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCAAACTCCCCAAACTC |
| Suggested primer 2: | GCGTTGTAGAGGCTGTAGGC |