| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065072 |
| Chromosome: | chromosome 2 |
| Location: | 7863544 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141700 | FAP315 | (1 of 2) PTHR12932//PTHR12932:SF9 - P25 ALPHA-RELATED // TPPP FAMILY PROTEIN CG45057; EF-Hand Containing Flagellar Associated Protein 315 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGCCCGGCAGCAAGCCAACGTCCGTGA |
| Internal bar code: | AGGATGCCATGACATCACTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 96.4464 |
| LEAP-Seq n confirming: | 3094 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGGTGTTGGGCTGATACT |
| Suggested primer 2: | GACACACTGGGGGCTACACT |