| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.065078 |
| Chromosome: | chromosome 2 |
| Location: | 991970 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080250 | YGG1,CGL97,YLMG1 | Conserved in the green lineage 97; (1 of 4) PF02325 - YGGT family (YGGT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGAGGTCCGGCCCGCAGGGGAAGTTGG |
| Internal bar code: | GAACGGGCAGTAGTTCTTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 196 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTCATGTTAGCCGTTGCC |
| Suggested primer 2: | CCACTGTTCGGTTTCCTCAT |