| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.065080 |
| Chromosome: | chromosome 16 |
| Location: | 4838252 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686203 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAACCGCAGCATCGGGAGCGACGCCGGC |
| Internal bar code: | TGAGAAGTCAGGGCGCGTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 39.6491 |
| LEAP-Seq n confirming: | 226 |
| LEAP-Seq n nonconfirming: | 344 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTGCTTTAACTCCCAAC |
| Suggested primer 2: | CCATATGTGTGCTTGGCAAC |