| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.065108 |
| Chromosome: | chromosome 16 |
| Location: | 264587 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694403 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCGGTCCACATGTCGATCGCTCCCTCG |
| Internal bar code: | TTAGCTGATCACATTGCGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 567 |
| LEAP-Seq percent confirming: | 99.0892 |
| LEAP-Seq n confirming: | 15013 |
| LEAP-Seq n nonconfirming: | 138 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCATGACACATGCACAC |
| Suggested primer 2: | CGGGAGTTTGTGTTTGTGTG |